Electron cryo-microscopy (cryoEM) is a rapidly expanding field that allows biological molecules to be imaged to resolve their 3-dimensional structure. Recently, the University of York has established a powerful Glacios electron microscope housed within a custom-designed building. We are beginning a program of further exploring the capabilities of the Glacios and optimising our experimental workflows. The successful candidate will work closely with academics, facility staff, and representatives from ThermoFisher Scientific, the microscope manufacturer. The project will focus on an emerging 3D imaging technique called electron tomography. Test samples will be imaged and the student will optimise our ‘subtomogram averaging’ computational pipeline to find homogeneous elements within them and average them together to decrease noise and improve resolution, allowing finder structural features to be resolved and interpreted. This project will provide in-depth training in operating an electron microscope and processing data in a cutting-edge and fast developing area. Students will be expected to present their findings orally at a research day in York in September 2023.