Prof. Sherif El-Khamisy, Biosciences, Sheffield
ATM is a key DNA damage response protein upregulated during the cell cycle, cancer drug treatment, and following exposure to UV rays. Angiogenesis is a key cellular process where surrounding tissue must be degraded to make space. One enzyme involved in this process is aminopeptidase-N (APN). Findings in our lab showed APN expression to be reduced in ATM knockout cells and literature indicates APN’s role in angiogenesis to be particularly important under hypoxic conditions. This raised the question, could APN be an intermediate step in an ATM-orchestrated hypoxia response? In this project, the effect of ATM depletion and overexpression on APN expression will be explored using an ATM knockout line, siRNA, and transient transfection. The effect on APN expression will be explored using western blotting and Image Lab and GraphPad Prism software will then be used to quantify and statistically analyse the data. Our lab is based at the University of Sheffield within the School of Biosciences. During their time here, the student will attend weekly lab meetings, as well as school-wide seminars. Students will be expected to present their findings orally at a research day in York in September 2023.