Cell differentiation: Effects on the therapeutic potential of extracellular vesicles

Extracellular vesicles (EVs) are an exciting new therapeutic modality with the potential to treat disease. EVs are nano-sized lipid vesicles containing a variety of bioactive cargo that mediate therapeutic effects in the target cell. The cell source and growth conditions are critical in determining the therapeutic properties of EVs. We have developed an immortalised mesenchymal stromal cell (MSC) line, that produces a consistent supply of therapeutically active EVs. This project will investigate the impact of MSC differentiation state on modifying the therapeutic properties of the EVs produced. The student will gain experience in cell culture techniques, bioprocessing through harvesting and purifying EVs, and also a range of different analytical techniques, such as nanoparticle tracking analysis and immunoblotting to characterise the EVs. Finally, EV bioactivity will be tested through robust cell signalling assays. As part of our vibrant research team, you will gain specialised experience in MSCs and EVs through a unique collaboration with our therapeutics spin-out company, Mesenbio. You will also have the opportunity to share your progress during regular lab meetings. Students will need to find their own accommodation and be expected to present their findings orally at a research day in York on 08th September 2026.